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DORFMAN Damian

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Título:

Enriched environment preserves the visual functions and reduces the structural damage of the optic nerve in an experimental model of optic neuritis

Autor/es:

ARANDA, MARCOS L; GONZÁLEZ FLEITAS, MARÍA F; DIEGUEZ, HERNÁN H; MILNE, GEORGIA A; DEVOUASSOUX, JULIÁN D; KELLER SARMIENTO, MARÍA I; CHIANELLI, MÓNICA S; SANDE PABLO H; DORFMAN, DAMIÁN; ROSENSTEIN, RUTH E

Lugar:

Buenos Aires

Reunión:

Congreso; Reunión Conjunta de Sociedades Biomédicas, LVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Resumen:

The therapeutic potential of enriched environment (EE) against damageinduced by neuroinflammation has been scarcely studied. Optic neuritis (ON) isan inflammatory, demyelinating and neurodegenerative disease of the optic nervethat can cause irreversible blindness. We studied the effect of EE housing onvisual pathway damage induced by experimental ON. Vehicle or LPS were micro-injectedinto the optic nerve of adult male Wistarrats. After injection, one group of animals was housed in EE and another groupremained in standard laboratory cages (SE) for 21 days. EE prevented ON-induceddecrease in consensual pupillary reflex (PR), visual evoked potential amplitude,and anterograde transport. Moreover, EE prevented the decrease in phosphorylatedneurofilament-immunoreactivity, microglial reactivity and astrocytosis, andreduced demyelination and prevented retinal ganglion cell and axon loss inducedby LPS injection (p<0.01). EE prevented the increase of oxidative damage andprotein levels of nitric oxide synthase-2 and cyclooxygenase-2, as well as thelevels of interleukin-1β mRNA and TNFαinduced by experimental ON (p<0.01). In addition, EE housing increasedbrain-derived neurotrophic factor levels (p<0.01). When EE exposure startedat 4 (but not 7) days post-injection of LPS, a protection of RP was observed at21 days post-LPS, which was blocked by daily administration of ANA-12 (a Trk-Breceptor antagonist) from day 4 to day 7 post-injection of LPS (p<0.01).Exposure to EE from day 4 to day 7 post-injection significantly preserved the PRat 21 days post-injection (p<0.01).These results indicate that EE housing reducesneuroinflammation of the optic nerve and preserves visual functions.

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