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DORFMAN Damian

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Título:

Enriched environment decreases retinal susceptibility to ischemic damage

Autor/es:

GONZÁLEZ FLEITAS, MARÍA F; DORFMAN, DAMIÁN; ARANDA, MARCOS L; DIEGUEZ, HERNÁN H; SANDE PABLO H; CHIANELLI, MÓNICA S; ROSENSTEIN, RUTH E

Lugar:

Baltimore

Reunión:

Congreso; ARVO Annual Meeting 2017; 2017

Resumen:

Purpose:Ischemia is a key component of several retinal diseases that are leading causesof irreversible blindness. At present, there are no effective strategies toprevent retinal ischemic damage. Enriched environment (EE) refers to conditionsthat facilitate or enhance sensory, cognitive, motor, and social stimulationrelative to standard (laboratory) conditions. The aim of the present work wasto analyze whether the exposure to EE prevents acute retinal ischemic damage.Methods:Adult male Wistar ratswere housed instandard environment (SE) or EE for 3 weeks. SE consisted instandard laboratory cages, housing 2 animals/cage, and EE consisted in bigcages housing 6 animals and containing several food hoppers, wheels anddifferent objects repositioned once/day and fully substituted once/week. In SEand EE, animals were provided with food and water adlibitum. After 3weeks of SE or EE, retinal ischemia was induced by increasing intraocularpressure above120 mm Hg for 40 min.Immediately after ischemia, both groups were further housed in SE for 2weeks.Retinal function (electroretinography, ERG),histology (H&E), Mullercells (glial fibrillary acidic protein, (GFAP immunoreactivity)) and retinalganglion cell (RGC) number(Brn3a immunohistochemistry) were assessed.Results:In animals housed in SE, ischemia induced a significant decrease in ERG a- andb- wave amplitude and oscillatory potentials, whereas the previous exposure toEE prevented these functional alterations. Two weeks after ischemia, asignificant decrease in the total retinal thickness and the number of Brn3a(+)cells, as well as an increase in Muller cell GFAP levels werefound in retinasfrom animals housed in SE, whereas in ischemic retinas from animals housed inEE,theseparameters were significantly preserved.Conclusions:These results suggest that the exposure to EE prevents retinal alterationsinduced by acute ischemia.

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