Impaired migration of CD8+ T cells and poor parasite control in target tissues of IL-10 deficient mice during acute Trypanosoma cruzi infection

MIRANDA, CRISTIAN GABRIEL ; PINO MARTÍNEZ, AGUSTINA ; BATALLA, ESTELA; GULIN, ERNESTO ; GONZÁLEZ CAPPA, STELLA MARIS; ALBA SOTO, CATALINA

Mar del Plata

Otro; Reunión Anual de Sociedades Biocientíficas.; 2019

SAIC-SAFE-SAB-SAP-AACYTAL-NANOMEDar-HCS

CD8+ T cells play a critical role limiting T. cruzi multiplication in peripheral tissues. To achieve this, they need to become functionally activated in lymphoid organs and sense environmental signals produced at the sites of inflammation to migrate, target and eliminate cells infected with intracellular amastigotes. We have previously established that IL-10 deficient mice (IL-10 KO) are more susceptible to infection with T. cruzi. Our findings demonstrated that splenic CD8+ T cells from IL10 KO infected mice fail to expand and to become fully activated thus suggesting an immunostimulatory role of this cytokine during acute T. cruzi infection. We hypothesize that impairment in CD8+ T cell activation has consequences on their migration and control of parasite replication in target tissues. Here, we analyzed the effects of absence of IL-10 in hearts of T.cruzi infected mice and particularly, we analyzed histologicalchanges, tissue infiltrating lymphocytes, parasite load as well as chemokines and chemokine receptors involved in migration of CD8+ T cells. After 21 days of infection tissue sections of hearts from IL-10 KO mice stained with hematoxylin and eosin exhibited a higher score of diffuse and perivascular infiltrates than WT mice. Correlating with the higher tissue inflammation, mRNA expression of chemokines CXCL9 and CXCL10 was upregulated in heart tissues of WT and IL-10 KO infected mice but to a greater extent in the last group (p