Effect of pharmacologic and genetic p300 modulation in the progression of hormone-independent breast cancer.

FERMENTO, M.E.; VILLEGAS, M.E.; FERRONATO, M.J.; GANDINI, N.A.; ALONSO, E.N.; FACCHINETTI,M.M.; CURINO, A.C.

Mar del Plata

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

SAIC

Breast cancer (BC) is a heterogeneous disease consisting of many subtypes that have different responses to treatment and different clinical outcomes, suggesting the need to find new molecular markers. Elsewhere we demonstrated that pharmacological inhibition of p300 acetylase function has an antitumoral role in murine hormone-independent (HI) BC LM3 cell line, and decreases tumor progression in a syngeneic murine model of LM3. In this work, we investigated the role p300 has on BC by performing pharmacological inhibition of p300 acetyl-transferase function and analyzing the effects on viability, migration, invasion and adhesion in human triple negative BC (TNBC) MDA-MB-231 cell line and on tumor progression in a xenograft murine model of MDA-MB-231. We observed that inhibition of p300 reduced viability (WST and cell count), migration (wound healing assay), invasion (Matrigel) and adhesion (cell count) in MDA-MB-231 cells (p