Mucins expression in colon carcinogenesis rodent model

LANG, C.; GANDINI, N.A.; CURINO, A.C; BERTÓ, P.; ULLUA, N.; MELATINI, G.; FERMENTO, M.E.; PÉREZ, J.E.

Rosario, Santa Fe

Congreso; XLII Reunión Anual - SAIB Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006

SAIB Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

MUCINS EXPRESSION IN COLON CARCINOGENESIS RODENT MODEL Lang, C.; Gandini, N.A.; Curino, A.C. ; Berton, P.; Ullua, N.; Melatini, G.; Fermento, M.E.; Pérez, J.E. Depto. Biología, Bioquímica y Fcia, UNS. Grupo Oncológico Cooperativo del Sur. INIBIBB. Bahía Blanca E-mail: anahis@uns.edu.ar Administration of 1,2-dimethylydrazine to rodents is known to cause adenocarcinomas of the colon similar to those seen in cancer patients, and it has been widely used as a colon cancer model. We have developed a similar animal model of hemicalinducedintestinal cancer for studying mucins expression. The mucins are secreted in normal/abnormal epithelium by the globet cells. Different studies have demonstrated a change in the transcript level as well as an increase in inmunoreactivity of the mucin along the colon carcinogenesis steps. The main objective of this study was to determine the secretion of sulphated acid mucin (SAM), non- sulphated acid mucin (NSAM) and neutral mucin (NM) in the lesions produced by 1,2-DMH in Wistar rats. Thirty animals were injected with 15 mg/kg of 1,2-DMH once a week during six consecutive weeks. Twenty-eight weeks after the first inoculation, rats were killed by CO2 asphyxiation. Then the mucin was studied with Alcian Blue 8GX (pH 1 and pH 2,5%) and peryodic acid shiff (PAS) staining. The SAM was found in 60% of the polyps and 40% of the malignant tumors. On the other hand, the NSAM was found in 55% of the polyps and in 46% of the malignant tumors. The NM was detected in 60% of the polyps and 40% of malignant tumors. No statistically significant difference was found in the secretion of NSAM, but we observed differences in the secretion of NM and SAM in both polyps and neoplastic tumors.