Arbuscular mycorrhizal fungal diversity in rhizosphere spores versus roots of an endangered endemic tree from Argentina: Is fungal diversity similar among forest disturbance types?

FLORENCIA SOTERAS; BRUNO C. MOREIRA; GABRIEL GRILLI; NICOLÁS PASTOR; FLÁVIA CARNEIRO MENDES; DANIELA RUELA DE CARVALHO; DANIEL RENISON; MARIA C. M. KASUYA; FRANCISCO DE SOUZA; ALEJANDRA G. BECERRA

APPLIED SOIL ECOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 98 p. 272 - 277

0929-1393

P { margin-bottom: 0.21cm; }The aim of this study was to comparethe arbuscular mycorrhizal fungal (AMF) community of the rhizosphereand inside the roots of the perennial Polylepis australistree. Three forest types differing in their structural complexitydue to anthropogenic disturbances were chosen at three differentsites at the high mountains of central Argentina. Rhizosphere sporesand P. australis roots of four randomly selected treeswere isolated from 36 soil samples, DNA was extracted and the 18SrDNA fragments were amplified by nested-PCR. The products wereanalyzed by DGGE and the bands were excised for sequencing. Intotal, 36 OTUs were defined from 56 DGGE bands successfullysequenced. Forest disturbance types showed similar communities ofAMF, as rhizosphere spores and within the roots of P.australis. However, DGGE clustering showed mainly differencesbetween rhizosphere spores and root-colonizing AMF. Members ofGlomeraceae, Pacisporaceae, Acaulosporaceae and Gigasporaceae wereshown in rhizosphere spore samples. Root samples showed only membersof Acaulosporaceae and Gigasporaceae, which might be complementary interms of soil resources exploration. The prevalence of the rootsystem with their community of symbionts might explain the resilienceof AMF soil communities to forests structural changes. This studypresents evidence of a possible preference in the AMF?P. australisinteraction.