INVESTIGADORES
MALAMUD Florencia
congresos y reuniones científicas
Título:
The role of flagella in motility and biofilm formation in Xanthomonas axonopodis pv citri.
Autor/es:
FLORENCIA MALAMUD; PABLO S TORRES; RIGANO L; CASTAGNARO AP; MARANO M.R.; VOJNOV AA
Lugar:
cancun
Reunión:
Congreso; ASM-biofilm; 2009
Resumen:
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Xanthomonas axonopodis pv. citri (Xac) is the causative agent of citrus canker, an important
threat to the citrus industry worldwide. Previous work from our laboratory
demonstrated that Xac is able
to form biofilm communities in biotic and abiotic surfaces where the
extracellular polysaccharide xanthan (EPS) plays a key role in the biofilm
maturation. Biofilm formation in other bacteria is a flagellar-dependent
process. Xac has a single polar
flagellum. To evaluate its participation in biofilm development we generated
two deletion mutants: Xac fliC (flagellin gene) and Xac flgE (hook gene), both involved
in the flagellar structure. Using the crystal violet technique we observed that
flagellar mutants were impaired in the first steps of biofilm development,
however, their ability to stick to the bottom of the Polystyrene-well was
partially recovered after 72 h. By confocal microscopy we observed that these
mutants were able to adhere but not to form a mature structure as wild type Xac did. We also show in this work
that Xac has the ability to
swim and to perform another type of movement that shows to be
flagellar-independent but EPS and quorum sensing-dependent. All mutants
affected in the flagellar-independent motility described here exhibit dispersal
growth without any cell-cell architecture in biofilms growth conditions. We
propose that this type of surface translocation is important for Xac at the adhesion step of biofilm
development and that the lack of flagellum was responsible for the impairment
of the mutants to develop a mature biofilm.