Transcriptome modulation of N. benthamina by Xanthomonas campestris pv. campestris DSF regulated factors

PABLO S TORRES; LUCIANO A RIGANO; FLORENCIA MALAMUD; ADRIAN A VOJNOV

Mar del Plata, Buenos Aires, Argentina

Congreso; SAIB; 2007

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES; mso-fareast-language:ES;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Xanthomonas campestris pv. campestris (Xcc) is a virulent pathogen that causes disease on crusiferous crops worldwide. The quorum sensing system mediated by rpf genes, the DSF (Difussible Signal Molecule) synthesized by RpfF protein, plays a key role in pathogenesis by modulation of wide range of  virulence factors (VF) that contributes to virulence and disease symptoms development. Recent studies suggest that glucan and xantan gum are involved in the suppression of host basal defense. However, little is known about the interplay between the host gene expression changes associated with basal defenses and the virulence activities of the  VF during infection. In this study, we used a c-DNA array representing 15000 genes of  Solanum tuberosum to determine the transcriptome associated with basal defense of Nicothiana benthamiana to Xcc rpfF mutant compared with Xcc 8004 (Wt.). Our global gene expression analysis identified 200 genes reproducibly regulated in response to VF regulated by DSF. Our results demonstrate that VF activate systemic immunity at the transcriptional level, and they provide insight into the coordinated transcriptional regulation of several plant defense genes.