Salmonella enterica serovar Enteritidis biofilm lifestyle induces lower pathogenicity and reduced inflammatory response in the murine model compared to planktonic bacteria.

GIACOMODONATO MONICA; SARNACKI SEBASTIAN; AYA CASTAÑEDA MARIA DEL ROSARIO; GAROFALO AILIN; BETANCOURT DIANA; CERQUETTI MARIA CRISTINA; NOTO LLANA MARIANGELES

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Año: 2022

0325-7541

Salmonella enterica serovar Enteritidis (S. Enteritidis) is the most frequent serovar involved in human salmonellosis. It has been demonstrated that about 80% of infections are related to biofilm formation. There is little information about pathogenicity of S. Enteritidis and its relationship to biofilm production. In this regard,this study was aimed to investigate the differential host response induced by S.Enteritidis biofilm and planktonic lifestyle. To this purpose, biofilm and planktonic bacteria were inoculated to BALB/c mice and epithelial cell culture. Survival studies revealed that biofilm is less virulent than planktonic cells. Reduced signs of intestinal inflammation and lower bacterial translocation were observed in animals inoculated with Salmonella biofilm compared to planktonic group. Results showed that Salmonella biofilm displays an impaired invasiveness of no phagocytic cells and induces a lower inflammatory response in vivo and in vitro compared to planktonic bacteria. Taken together, the outcome of Salmonella -host interaction varies depending on the bacterial lifestyle.