Probiotic characteristics and aflatoxin B1 binding ability of Debaryomyces hansenii and Kazaschtania exigua from rainbow trout environment

MARIA PÍA MARTINEZ; MARIA L, GONZALEZ PEREYRA; MARIA G FERNANDEZ JURI; VALERIA, POLONI; LILIA, CAVAGLIERI

AQUACULTURE RESEARCH

WILEY-BLACKWELL PUBLISHING, INC

Año: 2018

1355-557X

The aim of this study was to evaluate probiotic properties and the aflatoxin B1 adsorptionability of yeasts isolated from rainbow trout intestine and fish feed to assess theiruse in the formulation of feed additives. Growth at pH 2, bacterial pathogens inhibition,bacterial pathogens co-aggregation, autoaggregation, homologous and heterologousinhibition against lactic acid bacteria were evaluated. Moreover, aflatoxin B1adsorption was tested. All strains were able to maintain viable (107 cells/ml) at pH 2.All strains isolated from intestine were identified as Kazaschtania exigua, while strainsisolated from feed were all identified as Debaryomyces hansenii. Kazaschtania exiguaRC035 and RC037 showed the strongest antimicrobial activity while K. exigua RC037and RC038 were the most efficient co-aggregating bacterial pathogens. All strainsexhibited strong autoaggregation. None of the tested yeast strains showed homologousinhibition towards other yeasts and heterologous inhibition towards lactic acidbacteria strains. Debaryomyces hansenii RC031 demonstrated aflatoxin B1 adsorptioncapacity (21%). The results of the present study indicate that select strains of Kazaschtaniaexigua and D. hansenii showed potential to improve the health of rainbowtrout by inhibiting pathogens and binding AFB1 and their use as probiotics mayimprove the production of rainbow trout in aquaculture systems.