Aflatoxin B 1 adsorption/desorption dynamics in the presence of Lactobacillus rhamnosus RC007 in a gastrointestinal tract simulated model

FOCHESATO, A. S; CUELLO, D.; POLONI, V.; GALVAGNO, M. A.; DOGI, C. A.; CAVAGLIERI, L. R.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2018

1364-5072

Aims: 1) to determine the aflatoxin B1 (AFB1) adsorption and desorption dynamics in the presence of L. rhamnosus RC007 under simulated transit of AFB1 at each gastrointestinal tract (GIT - saliva, stomach and intestine) stage consecutively and then, separately, 2) to study the ability of L. rhamnosus RC007 to biotransform AFB1 as a strategy that complements the adsorption process. Methods and results: the AFB1 adsorption and desorption assay simulating the GIT passage of AFB1 (93.89 ng/g) in presence of L. rhamnosus RC007 (108 CFU/mL) was conducted. Moreover, lactic acid production was determined. Results demonstrated that predominant environmental conditions in salivary solution (SS) induced a low AFB1 adsorption, while the transit through the gastric solution (GS) and intestinal solution (IS) allowed high percentages of adsorption and did not generate significant AFB1 desorption. Conclusions: The AFB1 adsorption and desorption dynamics in the presence of L. rhamnosus RC007 was favoured by gastric and intestinal environment. Significance and impact of study: the knowledge of the adsorption dynamics of AFB1 with a microorganism of interest will allow predicting its behavior at each stage of the GIT.