A Saccharomyces cerevisiae RC016-based feed additive reduces liver toxicity, residual aflatoxin B1 levels and positively influences intestinal morphology in broiler chickens fed chronic aflatoxin B1-contaminated diets

VALERIA, POLONI; ALEJANDRA MAGNOLI; ANALIA, FOCHESATO; ANDREA CRISTOFOLINI; MATIAS CAVERZAN; CECILIA MERKIS; MARIANA, MONTENEGRO; LILIA, CAVAGLIERI

ARCHIVES OF ANIMAL NUTRITION

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2019 p. 31 - 38

1745-039X

The present study was conducted to investigate the ability of Saccharomyces cerevisiae RC016-based feed additive to reduce liver toxicity, residual aflatoxin B 1(AFB 1 ) levels and influence intestinal structure in broiler chickens fed on chronic aflatoxin B1 levels-contaminated diets. A total of day-old, one-hundred (100) male commercial line (Ross) broiler chickens were divided into 4 treatments, with 5 pens per treatment and 5 broiler chickens per pen. Birds were randomly assigned to 4treatments namely, T 1 : control diet (CD); T 2 : CD + S. cerevisiae RC016 (Sc) 1g/kg; T 3 : CD + AFB 1 (100 μg/kg); T 4 : CD + Sc 1 g/kg + AFB 1 (100 μg/kg).The liver histopathology of broiler chickens fed with AFB 1 showed diffused microvacuolar fatty degeneration. The addition of S. cerevisiae RC016 showed normal hepatocytes similar to the control. The small intestine villi from AFB 1 group showed atrophy, hyperplasia of goblet cells, prominent inflammatory infiltrate and o edema. In contrast, the small intestine villi from birds that received the yeast plus AFB1 showed an absence of inflammatory infiltrate, and atrophy; moreover, a lower number of goblet cells compared to the group with AFB 1 was observed. Themorphometric intestine studies showed that a significant decrease (P0.05), there was a tendency to improve these parameters. The residual levels of AFB 1 in livers were significantly reduced (P