Aflatoxin B 1 adsorption/desorption dynamics in the presence of Lactobacillus rhamnosus RC007 in a gastrointestinal tract-simulated model

FOCHESATO, A.S.; CUELLO, D.; POLONI, V.; GALVAGNO, M.A.; DOGI, C.A.; CAVAGLIERI, L.R.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2019 vol. 126 p. 223 - 229

1364-5072

Aims: (i) To determine the aflatoxin B 1 (AFB 1 ) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB 1 at each gastrointestinal tract (GIT?saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 to biotransform AFB 1 as a strategy that complements the adsorption process. Methods and Results: The AFB 1 adsorption and desorption assay simulating the GIT passage of AFB 1 (93·89 ng g −1 ) in the presence of L. rhamnosus RC007 (10 8 CFU per ml) was conducted. Moreover, lactic acid production was determined. Results demonstrated that predominant environmental conditions in salivary solution induced a low AFB 1 adsorption, while the transit through the gastric solution and intestinal solution allowed high percentages of adsorption and did not generate significant AFB 1 desorption. Conclusions: The AFB 1 adsorption and desorption dynamics in the presence of L. rhamnosus RC007 was favoured by gastric and intestinal environment. Significance and Impact of the Study: The knowledge of the adsorption dynamics of AFB 1 with a micro-organism of interest will allow predicting its behaviour at each stage of the GIT.