Imaging Golgi Outposts in fixed and living neurons

BISBAL M,; QUASSOLLO G,; CACERES A

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

Humana Press, USA / Springer publishing group

Año: 2016 vol. 1496 p. 31 - 39

1064-3745

Here we describe the use of confocalmicroscopy in combination with antibodies specific to Golgi proteins tovisualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidalneurons. We also describe the use of spinning disk confocal microscopy, incombination with ectopically expressed glycosyltranferases fused to GFPvariants, to visualize GOPs in living neurons.