INVESTIGADORES
BISBAL Mariano
artículos
Título:
Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia
Autor/es:
RIVERO MR., VRANYCH CV., BISBAL M., MALETTO BA., ROPOLO AS., TOUZ MC.
Revista:
BIOCHEMICAL JOURNAL
Editorial:
PORTLAND PRESS LTD
Referencias:
Lugar: Londres; Año: 2010 vol. 428 p. 33 - 45
ISSN:
0264-6021
Resumen:
The parasite Giardia lamblia possesses PVs (peripheral vacuoles) that
function as both endosomes and lysosomes and are implicated in the
adaptation, differentiation and survival of the parasite in different
environments. The mechanisms by which Giardia traffics essential
proteins to these organelles and regulates their secretion have
important implications in the control of parasite dissemination. In the
present study, we describe the participation of the heterotetrameric
clathrin-adaptor protein gAP2 (Giardia adaptor protein 2) complex in
lysosomal protein trafficking. A specific monoclonal antibody against
the medium subunit (g mu 2) of gAP2 showed localization of this complex
to the PVs, cytoplasm and plasma membrane in the growing trophozoites.
gAP2 also co-localized with clathrin in the PVs, suggesting its
involvement in endocytosis. Uptake experiments using standard molecules
for the study of endocytosis revealed that gAP2 specifically
participated in the endocytosis of LDL (low-density lipoprotein).
Targeted down-regulation of the gene encoding g mu 2 in growing and
encysting trophozoites resulted in a large decrease in the amount of
cell growth and cyst wall formation, suggesting a distinct mechanism in
which gAP2 is directly involved in both endocytosis and vesicular
trafficking.