ANDROGEN DEPRIVATION AND LUNG DISEASE RISK- THE ROLE OF NRG1 AND HO-1

BOLDRINI GABRIEL GIEZI; MARTIN MOLINERO GLENDA D; PIGUILLEM SILVANA; BIAGGIO VERONICA S; CIMINARI MARIA EUGENIA; ALVAREZ SILVINA M; PEREZ CHACA MARIA VERONICA; GOMEZ NIDIA NOEMI

Mendoza

Congreso; XXXIV Reunion Anual de la Sociedad De Biologia de Cuyo; 2016

Sociedad de Biologia de Cuyo

Previously, we demonstrated a change of pro-inflammatory parameters in lung parenchyma after castration. The antioxidant responseelements (ARE) are involved in the activation of genes coding for a number of antioxidant proteins. Androgen deprivation triggers of oxidative stress and exacerbates lung injury. We studied if castration produced changes in oxidative stress markers and inflammation parameters. Male Wistar rats (200 ± 20 g) were separated in 3 lots: controls (Co), castrated (Ca), and castrated supplemented with testosterone (Ca+T) for five days. 60 days after castration rats were killed and lungs were obtained. Before that, bronchoalveolar lavages (BAL) were collected and cell count was performed.RNA was extracted by using TRIzol. mRNA levels of TGF-β, HO-1, Neuregulin (NRG-1), VCAM, COX2 and FOXO3a were quantified by RT-PCR. ANOVA was used for statistical analysis. After 60 days of castration, COX2 significantly increased its expression in Ca group (p