THE PROTECTIVE ROLE OF A SOYBEAN DIET IN CEREBELLUM OF CADMIUM INTOXICATED RATS

MARTIN MOLINERO GLENDA D; BOLDRINI GABRIEL GIEZI; FILIPPA VP; PEREZ CHACA MV; GOMEZ NN; GIMENEZ MS; ALVAREZ SM

Congreso; XXXVI Reunión Anual de la Sociedad de Biologia de Cuyo; 2018

Sociedad de Biologia de Cuyo

Cadmium is a toxic agent and is also an environmental contaminant. We studied its effects and the protective role of a vegetarianversus animal protein diet in cerebellum. Histoarchitecture, morphometric parameters, cadmium concentration, antioxidantenzyme activities and oxidative stress markers were determined. Female Wistar rats were used; 2 lots received casein (Cas) and 2lots soybean (Soy) as protein source (n=6). Within each group, 1 lot received regular water (Co) and the other, 15 ppm of Cd for60 days. NF-E2 related nuclear factor (Nrf-2), SOD 2, CAT, GPx, iNOS, VCAM-1 mRNAs were determined by RT-PCR. S28was used as internal control. Histological studies were performed in H&E stained tissues. Morphometric parameters weremeasured in ten random visual fields in four sections (X40) and were analyzed with IMAGE J Software. Cadmium concentrationwas determined with ICP-MS. CAT and GPx activities were measured in cerebellum homogenates. Nrf-2 decreased in both Cdgroups and in Soy Co (p