RHO KINASE ATTENUATES CALCIUM-INDUCED CONTRACTION IN B-ESCIN BUT NOT TRITON X-100 PERMEABILIZED RABBIT FEMORAL ARTERY

CLELLAND L; BROWNE B; MINER AS; ALVAREZ SM; RATZ PH

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY

SPRINGER

Año: 2011

0142-4319

K?-depolarization (KCl) of smooth muscle has long been known to cause Ca2?-dependent contraction, but only recently has this G protein-coupled receptor (GPCR)- independent stimulus been associated with rhoA kinase (ROCK)-dependent myosin light chain (MLC) phosphatase inhibition and Ca2? sensitization. This study examined effects of ROCK inhibition on the concentration–response curves (CRCs) generated in femoral artery by incrementally adding increasing concentrations of KCl to intact tissues, and Ca2? to tissues permeabilized with Triton X-100, b-escin and a-toxin. For a comparison, tissue responses were assessed also in the presence of protein kinase C (PKC) and MLC kinase inhibition. The ROCK inhibitor H-1152 induced a strong concentration-dependent inhibition of a KCl CRC. A relatively low GF-109203X concentration (1 lM) sufficient to inhibit conventional PKC isotypes also inhibited the KCl CRC but did not affect the maximum tension. ROCK inhibitors had no effect on the Ca2? CRC induced in Triton X-100 or a-toxin permeabilized tissues, but depressed the maximum contraction induced in b-escin permeabilized tissue. GF-109203X at 1 lM depressed the maximum Ca2?-dependent contraction induced in a-toxin permeabilized tissue and had no effect on the Ca2? CRC induced in Triton X-100 permeabilized tissue. The MLC kinase inhibitor wortmannin (1 lM) strongly depression the Ca2? CRCs in tissues permeabilized with Triton X-100, a-toxin and b-escin. H-1152 inhibited contractions induced by a single exposure to a submaximum [Ca2?] (pCa 6) in both rabbit and mouse femoral arteries. These data indicate that b-escin permeabilized muscle preserves GPCR-independent, Ca2?- and ROCK-dependent, Ca2? sensitization.