CHARACTERIZATION OF TONSILLAR IL10 SECRETING B CELLS AND THEIR ROLE IN THE PATHOPHYSIOLOGY OF TONSILLAR HYPERTROPHY

LINDYBETH SARMIENTO; JAVIER DE ROSA; ARIEL BILLORDO; PLÁCIDA BAZ; ANDRÉS BLANCO; PABLO FERNANDEZ; ELOÍSA ARANA

Buenos Aires

Congreso; LXV Reunión Anual de la Sociedad Argentina de Inmunología; 2017

The immune actions of tonsillar B cells (Bc) must be tightly regulatedto balance the protection against virulent germs and the toleranceto harmless flora and innocuous Ags in food and air. Despitethe logic of such presumption, the potential role of tonsils in oraltolerance induction has not been completely elucidated so far. Theaim of our work was to test the ability of tonsillar Bc to secrete IL10(regulatory cytokine) and to assess the physiological relevance ofthis population. All measurements were done by FACS. We determinedthe phenotype of tonsillar IL10 secreting Bc (tBregs). Also,we found that the frequency of tBregs as determined by FACS upon72 hs stimulation ex vivo, via TLR9 and CD40L, depended on thecause of surgery. The hypertrophied (HT) tonsils´ samples showeda significantly (p