Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis and Malassezia globosa to azole drugs and amphotericin B evaluated by a broth microdilution method

ROJAS F; SOSA MA; FERNÁNDEZ M; CATTANA ME; CORDOBA S; GIUSIANO G

MEDICAL MYCOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2014 vol. 52 p. 641 - 646

1369-3786

We studied thein vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenicMalasseziaspecies,M. globosa,M. sympodialis,andM. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32o C. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialiswas the most susceptible andM. furfurthe least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility ofMalasseziaspecies. Further investigations and collaborative studies are essential for correlatingin vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.