Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V

TRIBULO A; ROGAN D; TRIBULO H; TRIBULO R; ALASINO RV; BELTRAMO DM; BIANCO ID; MAPLETOFT RJ; BO GA

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011 p. 7 - 13

0378-4320

The need to inject FSH twice daily for superstimulation of ovarian follicular development in cattle necessitates frequent attention by farm-personnel and increases the possibility of failures due to mishandling and errors in administration of treatments. A series of three experiments were designed to evaluate the feasibility of superstimulation in beef cattle with a single intramuscular (IM) injection of Folltropin-V diluted in a hyaluronan-based slow-release formulation (SRF). In Experiment 1, cows were assigned to one of three treatment groups to compare two methods of injection as compared to the twice daily IM injections over 4 days (Control) was greater than of cows treated with a single subcutaneous (SC) injection in SRF (n=6), while superovulatory response of cows treated witn a single IM injection in SRF (n=6) was intermediate. Experiment 2 was designed to compare two concentrations of  SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL) were not significantly different (P¡Ý0.05), but the numbers of total ova/embryos (P¡Ý0.05), fertilized ova (P¡Ý0.01) and transferable embryos (P¡Ý0.001) were greater in cows treated with FSH in 100% SRF (n=20) than cows treated with FSH in 50% SRF (n=20). Experiment 3 was designed to compared superovulatory response in Red Angus donor cows treated with a single IM injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional twice-daily IM injection protocol over 4 days. Mean (¡ÀSEM) numbers of CL (13.7¡À1.2 compared to 13.8¡À1.), total ova/embryos (12.3¡À1.5 compared to 13.7¡À2.1), fertilized ova (7.2¡À1.1 compared to 8.4¡À1.4) and transferable embryos (4.9¡À0.8 compared to 6.4¡À1.3) were not significantly different between Control (n=29) and Single injection (n=29) groups, respectively.