INVESTIGADORES
AMARAL Maria Marta
congresos y reuniones científicas
Título:
A globotriaosylcramide inhibitor prevented the effect of Shiga toxin 2 on cell proliferation and migration in renal tubular epithelial cells
Autor/es:
DAIANA SANCHEZ; LILIAN KARINA FISCHER SIGEL; CRISTINA IBARRA; MARIA MARTA AMARAL; CLAUDIA SILBERSTEIN
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018
Institución organizadora:
SAIC-SAI-SAFIS
Resumen:
In Argentina, post-diarrhea Hemolytic Uremic Syndrome (HUS) dueto Shiga toxin-producing Escherichia Coli (STEC) is a commoncause of acute renal failure in children. Shiga toxin type 2 (Stx2)binds to the globotriaosylceramide (Gb3) receptor on the surface ofrenal cells. We have previously shown that the compound C-9 (Genzyme), an inhibitor of glucosylceramide synthase, decreased Gb3expression in renal epithelial cells. The aim of this work was to studywhether the C-9 prevents the cytotoxic actions of Stx2 on cell proliferation and cell migration in Vero cultures. Cell proliferation wasmeasured by bromodeoxyuridine (BrdU) uptake, and cell migration was evaluated as the percentage of wound closure in wound healingassay. Incubation of Vero cultures with Stx2 (10 nM, 24 h) significantly decreased the BrdU uptake to 48 ± 18 % (p
