Quantitative alterations in splenic dendritic cell subsets, T regulatory cells and cell proliferation in infected or vaccinated mice with foot-and-mouse disease virus

LANGELLOTTI, CECILIA; QUATTROCCHI, VALERIA; AMARAL MARÍA M; ALVAREZ CAROLINA; PEREYRA, ERICA; ZAMORANO, PATRICIA; VERMEULEN, MÓNICA

Buenos Aires

Congreso; First French - Argentine Immunology Congress LVIII Reunión Anual de la Sociedad Argentina de Inmunología. XIII Jornada Científica del Grupo Rioplatense de Citometría de Flujo. 3º Jornadas Argentinas de Inmunodeficiencias Primarias (SAP); 2010

Sociedad Argentina de Inmunología. French Society of Immunology. Grupo Rioplatense de Citometría de Flujo. Sociedad Argentina de Pediatría.

Foot-and-mouth disease (FMD) is a highly contagious, acute and feverish viral disease of cloven-hoofed animals. Previously, we have demonstrated that vaccination with inactivated FMDV induces an increase in plasmacytoid dendritic cells, in contrast with the infection that induces a decrease in this subset. Now, we studied the possible alterations in different subsets of dendritic cells (DC) and T lymphocytes between vaccination and infection. For that, we used adult Balb/c mice to infected i.p. with FMDV or vaccinated. Control mice were inoculated with supernatant of uninfected BHK-21 cell cultures. After, 1 or 3 days we obtained the population of splenic DC by using anti-CD11c/anti-mPDCA-1 magnetic beads. We found that the proportion of different DC subsets changes among vaccination and infection. While in vaccination myeloid DC (CD11c+/CD8a-) decreased significantly and lymphoid (CD11c+/CD8a+) DC mantained the same proportion as the control, in case of infected mice both myeloid (% of positive cells mean±SEM, Ct:28.24±3.18; iFMDV:20.96±2.43 ; FMDV:14.41±2.07, *p