Neutrophil-Derived Circulating Free DNA (cf-DNA): an associated marker for Hemolytic Uremic Syndrome development.

RAMOS, MARÍA V; MEJÍAS, PILAR; SABBIONE, FLORENCIA; ABREY-RECALDE, JIMENA; FERNÁNDEZ BRANDO, ROMINA; AMARAL MARÍA M; EXENI, RAMÓN; TREVANI ANALÍA; PALERMO MARINA S

Mar del Plata

Congreso; LVII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA LX REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA - SAI; 2014

SAIC-SAI

Hemolytic Uremic Syndrome (HUS), a vascular disease characterized by hemolityc anemia, thrombocitopenia and acute renal failure is caused by enterohemorraghic Shiga toxins (Stx)-producing bacteria. Besides Stx, inflammatory response mediated by neutrophils (PMN) is essential to HUS evolution. PMN have the capacity to release "neutrophil extracellular traps" (NETs) which are involved in the pathogenesis of several diseases. NETs are composed of neutrophil-derived circulating free DNA (cf-DNA), histones and PMN proteins such as proteases. The aim of this work was to investigate the presence of cf-DNA in HUS patients. We observed increased values of cf-DNA in plasma from HUS compared to healthy children (HC) ((HC:5,1±0,5; HUS:10±0,5*)ug/ml, n=15, *p