NEW INSIGHTS IN THE REGULATION OF THE RECRUITMENT OF ΓΔ T LYMPHOCYTES BY HUMAN GLOMERULAR ENDOTHELIAL CELLS EXPOSED TO SHIGA TOXIN TYPE 2

DAVID ANTONIO ROSSO; FERNANDO DANIEL GÓMEZ; NADIA CAROLINA MELILLO; MICAELA ROSATO; CRISTINA IBARRA; MARÍA MARTA AMARAL; CAROLINA CRISTINA JANCIC

Mar del Plata

Congreso; Reunión de Sociedades de Biociencias 2022; 2022

SAIC. SAFIS. SAI-FAIC.

Hemolytic uremic syndrome (HUS) is a late acute onset of symptoms that can occur after an intestinal infection with Shiga toxin (Stx)-producing Escherichia coli (STEC). STEC strains expressing Stx2a are the most common etiologic pathogen responsible for severe cases of HUS in Argentina. The immune system participates during the development of HUS by promoting inflammation and exacerbating the renal tissue damage, initially caused by the Stx. γδ T cells are a subset of T lymphocytes that act as early sensors of cellular stress and infection. They infiltrate the tissues and, in kidney injury, γδ T cells can potentially be recruited as they express the fractalkine receptor (CX3CR1), which is involved in the homing of NK cells and monocytes in the kidney. Previously, we demonstrated that conditioned medium derived from Stx2a-treated human glomerular endothelial cells (HGEC), activated γδ T lymphocytes and induced a Th1-like profile. In this work, our aim was to evaluate whether those conditioned media could modulate γδ T cell motility. To do this, we analyzed cell migration using the Transwell system and F-actin polymerization in γδ T cells, by flow cytometry. For this, the cells were exposed to conditioned media derived from HGEC treated with Stx2a (0.01 ng/mL, 24 h) or untreated ones. As results, we found that Stx2a-treated HGEC conditioned medium increased γδ T cell migration compared with culture medium alone (p