Ureide Permeases 5 (AtUPS5) connects compartments involved in ureide metabolism.

CARLOS IGNACIO LESCANO; FLORENCIA BOGINO; CAROLINA MARTINI; TOMÁS TESSI; CLAUDIO GONZÁLEZ; KARIN SCHUMACHER; MARCELO DESIMONE

Córdoba

Congreso; XXXII Reunión Argentina y XVI Congreso Latinoamericano de Fisiología Vegetal.; 2018

SAFV

The transport of metabolites plays an important role for optimal plant adaptation to the environment. Allantoin is a purine metabolite that was recently related with stress tolerance in plants. The subcellular localization of enzymes that lead to allantoin synthesis suggest that allantoin is produced in peroxysomes and loaded to vesicles for export via the secretory pathway, although the transport system remains still unidentified. AtUPS5 (Arabidopsis thaliana Ureide Permease 5) is a transmembrane protein that transports allantoin with high affinity. We studied the subcellular fate of splicing variants AtUPS5L (Large) and AtUPS5s (short) by tagging them with fluorescent proteins in their central loops. The capability of these fusion proteins to correctly complement the function of native ones was proved by nutritional and salt stress experiments. Both variants were localized in the endoplasmic reticulum, but the AtUPS5L variant was also localized in the trans-Golgi network/early endosome (TGN/EE). The subcellular fate of AtUPS5 splicing variants suggest that they could have different roles in allantoin distribution between subcelular compartments in the plant cells.