Lotus japonicus plants of the Gifu B-129 ecotype subjected to alkaline stress improve their Fe2+ bio-availability through inoculation with Pantoea eucalypti M91

CAMPESTRE MP, CASTAGNO LN; ESTRELLA MJ; RUIZ OA

JOURNAL OF PLANT PHYSIOLOGY

ELSEVIER GMBH

Año: 2016

0176-1617

Inoculation assays with P. eucalypti M91 were performed on L. japonicus ecotype Gifu. Under alkaline conditions, this ecotypeis characterized by the development of interveinal chlorosis of the apicalleaves due to low mobilization of Fe2+. Inoculation with P.eucalypti M91, a plant growth-promoting bacterial strain capable ofproducing pyoverdine-like and pyochelin-like siderophoresunder alkaline growth conditions, alters the root, resulting in a herringbonepattern of root branching. Additional features include improvement in Fe2+transport to the shoots, acidification of the hydroponic solution of the plantcultures, and an accompanying increase in the efficiency of the PSIIparameters. In addition, there was an increase in the expression of the FRO1 and IRT1 genes, accompaniedby a significant increase in FRO activity. Results showed that P. eucalypti M91 has a beneficial effect onthe Fe acquisition machinery of Strategy I, as described for non-graminaceousmonocots and dicots, suggesting its potential as an inoculant for legume cropscultivated in alkaline soils.