Responses of branchial biomarkers of Prochilodus lineatus after prolonged exposure to sublethal concentrations of fipronil

SANTILLAND DEIU A.; ONDARZA P.M.; MIGLIORANZA K.S.B.; DE LA TORRE F.

San Pablo

Congreso; 12th Biennial Meeting Society of Environmental Toxicology and Chemistry; 2017

Society of Environmental Toxicology and Chemistry Latino América

Fipronil (Fp) is a broad-use pesticide, and exhibits severe toxicity and adverse effects to non-target organisms, including fish. In this context, the presence of biochemical responses in fish is an effective tool for detecting sublethal effects from pesticide exposure. The responses of branchial biomarkers were evaluated on juveniles of Prochilodus lineatus after prolonged exposure to sublethal concentrations of Fp. Fish (n=32) were exposed to two nominal concentration of Fp (0.5 µg/L, C1 and 9 µg/L, C2) where toxicant was dissolved in methanol. Fish were kept in controlled conditions in aquariums with daily renewal of the media. Control fish remained in unchlorinated water (Ctrl) and water with the maximum methanol concentration used (CtrlSv). Levels of Fp on water samples were also analyzed with liquid-liquid and GC-ECD extraction. After 21 days of exposure, fish were sacrificed and the branchial arches were extracted.Enzymatic activity of superoxide dismutase (SOD), antioxidant capacity against peroxyles (ACAP), content of reduced glutathion (GSH), levels of lipid peroxidation by TBARS as well as determination of oxidatively modified proteins (POx) were evaluated. Statistical differences between groups were determined using ANOVA (p