Sulfated arabinogalactans from green marine algae showed anti-herpetic activity without eukaryotic cytotoxicity

MATOS, MARINA; PUJOL, CARLOS; FERNÁNDEZ, PAULA VIRGINIA; ARATA, PAULA XIMENA; CIANCIA, MARINA

San Martín

Simposio; 3º Simposio Argentino de Glicobiología; 2019

CONICET - UNSAM - FIL

Sulfated polysaccharides are interesting blanks to look forbiological activities with application in human health. Most ofstudies were focused on red algae. In this work, seven sulfatedarabinogalactans with different structural features obtained fromaqueous extracts form green algae were studied: Cv1 and Cv2from Codium vermilara, Cs1 and Cs2 from Codiumsubantarcticum, Cf1 and Cf2 from Cladophora falklandica, andPc1 from Penicillus capilatus. None of them showed cytotoxicactivity against VERO cells, even at 1 mg/ml. The ability offractions to inhibit VERO cell infection by Herpes Simplex VirusHSV-1 and HSV-2 was analyzed. Fractions showed a broadspectrum of inhibitory activity: Cs1 and Cv2, low; Cf2 and Cs2,moderate (IC50 112 µg/ml and 194 µg/ml); Cf1 and Pc1, higher(IC50 33 µg/ml and 44 µg/ml), while Cv1 showed the highestactivity (IC50 7 µg/ml). The major structure in Cv1 was a highlysulfated pyranosic 3-linked β-L-arabinan, while CF1 was also asulfated pyranosic β-L-arabinan, but the arabinose units were 4-linked and, in spite of its higher sulfate content, it was less active.This could be due to a significant amount of side chains,comprising mostly single stubs of β-D-xylose linked to C2. Thisand other structure-activity considerations, show the importanceof many structural details on the activity, not restricted to sulfatecontent and monosaccharide composition, but also glycosidiclinkage types, sulfation sites, backbone ramifications, and so on.