Glucuronoarabinoxylans as major cell walls polymers from youngshoots of the woody bamboo Phyllostachys aurea

ZELAYA, VÍCTOR MARTÍN; FERNÁNDEZ, PAULA VIRGINIA; VEGA, ANDREA S.; MANTESE, ANITA IDA; FEDERICO, ANA AILÉN; CIANCIA, MARINA

CARBOHYDRATE POLYMERS

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017

0144-8617

Young shoots of Phyllostachys aurea showed glucuronoarabinoxylans (GAX) as the major hemicellulosiccomponents, being extracted in major amounts with 1 M KOH (ratio Xyl:Ara:GlcA, 100:67:8), but also with water, showing a broad structural variability. Mixed linkage glucans were also present, but in minor amounts, mostly concentrated in the 4 M KOH extracts, while pectin polymers were very scarce. Arabino-galactan proteins were an important part of water extracts, determined by the presence of the typical arabinogalactan structures (3- and 6-linked Gal p; terminal and 5-linked Ara f), in addition to small amounts of hydroxyproline (2-3% of total protein) and positive reaction to Yariv?s reagent. Morphological and anatomical characteristics of young shoots are described, as well as localization of some cell wall components, and related with chemical analysis. A method for determination of uronic acids as their N-propylaldonamide acetates and separation and quantification by GC/MS was adapted for its use with grass cell wall fractions.