INVESTIGADORES
FERNANDEZ Paula Virginia
artículos
Título:
Potato Snakin-1 gene silecing affects cell division, primary metabolism and cell wall composition
Autor/es:
NAHIRÑAK, V.; ALMASIA, N.I.; FERNÁNDEZ, PAULA VIRGINIA; HOPP, E.H.; ESTEVEZ, JOSÉ MANUEL; CARRARI, F.; VAZQUEZ-ROVERE, C.
Revista:
PLANT PHYSIOLOGY.
Editorial:
AMER SOC PLANT BIOLOGISTS
Referencias:
Año: 2012 vol. 158 p. 252 - 263
ISSN:
0032-0889
Resumen:
Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a
transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1
silencing resulted in reduced height, which was accompanied by an
overall reduction in leaf size and severe alterations of
leaf shape. Analysis of the adaxial epidermis of
mature leaves revealed that silenced lines had 70% to 90% increases in
mean
cell size with respect to wild-type leaves.
Consequently, the number of epidermal cells was significantly reduced in
these
lines. Confocal microscopy analysis after
agroinfiltration of Nicotiana benthamiana leaves
showed that SN1-green fluorescent protein fusion protein was localized
in plasma membrane, and bimolecular fluorescence
complementation assays revealed that SN1
self-interacted in vivo. We further focused our study on leaf metabolism
by applying
a combination of gas chromatography coupled to mass
spectrometry, Fourier transform infrared spectroscopy, and
spectrophotometric
techniques. These targeted analyses allowed a
detailed examination of the changes occurring in 46 intermediate
compounds from
primary metabolic pathways and in seven cell wall
constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense.