Synthesis of aminated magnetite nanoparticles and their interaction with biomembrane models

SALAS, SANTIAGO D.; FERREYRA, NANCY F.; VICO, RAQUEL V.

Otro; 13th Postgraduate Summer School on Green Chemistry 2021; 2021

The widespread applications of magnetic nanoparticles (MNP) in i.e. biomedicine, sensing, and environmental remediation have increased in the last years. However, the dissemination of these novel materials in the environment and their potential toxicity have become an issue of major concern. The first natural barrier that NP find when they interact with living cells is the cellular membrane. The study of the effects of NP in organisms and cells can be complex, though, due to difficulties in the control of several variables. In this sense, the application of simplified and controlled biomembrane models, such as Langmuir monolayers, can be accurate to elucidate the molecular and supramolecular mechanisms that modulate NP-biointerface interaction and determine the mechanisms of toxicity. In previous work, we have synthesized different types on NP with a wide range of capping agents, and we assessed their interactions con biomembrane models using Langmuir monolayers comprising different types of lipids. [1][2]In this work we show the synthesis of magnetite (Fe3O4) MNP capped with an aryl diazonium salt (MNP@Ar-NH2) [3] (Figure 1), followed by cross-linking with glutaraldehyde (GA) (MNP@GA), and then, a Schiff base formation with branched polyethylenimine (PEI) to amplify amine groups on the surface (MNP@GA@PEI) [4]. TEM, XRD, TGA, zeta potential and hydrodynamic radii vs pH, FT-IR, and amine quantification confirm the presence of the new functional groups after each capping step. Furthermore, we will assess the effect of MNP@GA@PEI concentration and charge on the interaction with a dimyristoylphosphatidylcholine (DMPC) monolayer on an aqueous subphase as a biomembrane model using a Langmuir minitrough.References[1] M.E. Villanueva et al. J. Colloids Interf. Sci. 2019, 543, 247-255. [2] J.V. Maya Girón et al. Environm. Science: Nano 2016, 3, 462-472.[3] N. Griffete et al. Colloids and Surfaces A: Physicochem. Eng. Aspects 2012, 415, 98-104.[4] T. Xia et al. Colloids and Surfaces A: Physicochem. Eng. Aspects 2014, 443, 552-559.