PPCPs accumulated mass in Typha latifolia (cattail) as a bioaccumulated metric, and their relation with the exposure time, water transpired and physicochemical properties.

PÉREZ, DÉBORA JESABEL; LOMBARDERO, LUCAS RODRIGO; DOUCETTE, WILLIAM JOSEPH

Mar del Plata

Congreso; VIII Congreso SETAC Argentina, Mar del Plata, 7- 11 Marzo, Modalidad Híbrida (presencial - virtual).; 2022

SETAC Argentina

The ubiquitous occurrence of Pharmaceutical and Personal Care Products (PPCPs) in aquatic environments is a worldwide environmental problem, which forces to think about comprehensive mitigation actions. Wetland plants results an excellent green management option to remove PPCPs. The PPCPs bioaccumulated mass (PPCPsMASS) is an appropriated metrics of plants accumulation capacity. We aimed to study PPCPsMASS in Typha latifolia tissues after long-term exposure and its relationship with time, transpiration and physico-chemical properties, as lipophilicity (LogKow), ionization behavior (pKa) and distribution coefficient (LogDow). Hydroponically grown plants (18 control and 18 exposed) were exposed to carbamazepine (CBZ), fluoxetine (FLX), gemfibrozil (GBZ) and triclosan (TRI) at environmentally relevant concentrations (20 μg/L each one) over 42 days. Plants were harvested at 7, 14, 21, 28, 35 and 42 days and separated into roots, rhizome, sprouts, stem and leaf sections (lower, middle and top), and dry tissue weights and PPCPs tissue concentrations were determined. PPCPMASS was calculated as the ratio between tissue concentration and dry-mass. Two-ways ANOVAs were applied to determine differences between exposure time and tissues. Principal component analysis was applied to study the correlations between PPCPsMASS, exposure time, transpiration and physico-chemical properties. CBZ, FLX and TRI were detected in all analyzed tissues, while GBZ was only detected in roots and rhizomes. The PPCPsTOTAL-MASS in roots and leaf was higher than 70 µg over 42 days. In rhizome, stem and sprouts, the PPCPsTOTAL-MASS was less than 10 µg. In root, TRIMASS contributed in more than 60%, while in leaf CBZMASS and FLXMASS the contribution surpassed 90% of the PPCPsTOTAL-MASS. In lower and middle leaf FLXMASS surpassed 50%, while in top leaf CBZMASS reach more than 90%. PPCPsROOT-MASS and PPCPsRHIZOME-MASS were strongly positively correlated with LogKow and LogDow. PPCPsLEAF-MASS was correlated with the exposure time, transpiration and pKa. We discussed the results in base of, the implications about to use the PPCPsMASS in plants as a bioaccumulation metric than the standard descriptors (i.e. concentrations, bioaccumulation factors), and the root taken-up, translocation, distribution in the body plant of PPCPsMASS in T. latifolia as a dynamic process strongly associated with the plant development and tissue functions, and its relation with the physico-chemical and biological parameters.