Expression of receptors for TGF-B Superfamily in cows with spontaneous and ACTH-induced cystic ovarian disease.

MATILLER, VALENTINA; HEIN, GUSTAVO J.; AMWEG, AYELEN N.; RODRIGUEZ, FERNANDA M.; BELOTTI, EDUARDO M.; QUERCIA E.; REY, FLORENCIA; SALVETTI, NATALIA R.

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). XLVIII Reunión Científica Anual de la Sociedad de Farmacología Experimental (SAFE).; 2016

Preliminary results shows significant changes in theexpression of components of the TGF-B isoforms TGF-B1, 2 and 3, and inhibin /activin / follistatin system in ovarian structures of dairy cattle with spontaneousand ACTH-induced cystic ovarian disease (COD). Given the evidence of its roleas an important molecules in paracrine and autocrine signaling pathways thatregulate the growth of ovarian follicle, and knowing that this components carryout their actions through binding to two types of membrane receptors, theobjective of the present work was examine the expression of TGF-B receptor (TGF-BR)I, TGF-BRII, TGF-BRIII, Activin Receptor (ACVR)IB and ACVRIIB in ovaries ofcows with COD previously evaluated for their related ligands. We worked withsections obtained from bovine ovaries with spontaneous COD (n=10), ACTH-inducedCOD (n=10), and controls ovaries (n=10). In 4 um thick sections, the presence andexpression of receptors was determined by indirect immunohistochemistry ingranulosa and theca cells. Type I receptors expressed higher staining (p<0.05) in tertiary follicles of spontaneous COD group than controlsfollicles, and type II receptor ACVRIIB, showed higher immunostaining in thecacells of spontaneous COD group than control. Here we detected changes in the expressionof the receptors necessary for the response to the growth factors of TGF-Bfamily in the COD cow?s ovaries in relation to the controls. The highexpression of all receptors analyzed in follicular cysts could belinked to the persistence over time of these structures. We can concludethat alterations found at ligands level materialize a change in signalling favouringthese actions at the cellular level. Thus, to unravel intraovarian signalingpathways, we postulate data that may contribute to the knowledge of thepathogenesis of COD and that can be applicable to the design of protocols andtherapeutic measures.