Altered chemotaxis and mononuclear cell infiltration in bovine ovary after ACTH stimulation.

ETCHEVERS, L; STASSI, AF; CABAÑA, E; ANGELI, E.; DÍAZ, P.U.; REY, F; SALVETTI, NR; ORTEGA, HH; AMWEG, AN

Congreso; LXVI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2021

Upon LH stimulation, the ovary exhibits signs of local inflammation: chemokine and cytokine release, vasodilation, leukocyte infiltration. Abnormal immune responses in ovary often resulted in reproductive failures. However, the effects of adrenocorticotropin (ACTH) on the complexity of this inflammatory response are not yet well characterized. The aim of this study was to evaluate the effect of ACTH during preovulatory period on the number of mononuclear cells (MCs) infiltrated in the ovary and on the mechanisms associated with cell infiltration. ACTH (100 IU) was administered to Holstein cows (n=11) during proestrus every 12 h for four days before ovulation when ovariectomy was performed (day 18). Daily ovarian Doppler ultrasonography was used to evaluate the percentage of irrigated area (IA), the pulsatility and resistance index on the dominant follicles. Also, blood sample was taken on day 18 to analyze systemic alterations on MCs using the hematology analyzer BC-2800. Macrophages (CD14), T (CD2) and B (CD79) lymphocytes distribution in ovary was analyzed by immunohistochemistry. In follicular wall samples, gene expression of chemokines MCP1, IL-8, CXCL1, CCL25 were evaluated. Additionally, IL-17a expression was analyzed by western blot. A Generalized Linear Model was used to analyze the results. The number of systemic monocytes and lymphocytes were similar between groups (p>0.05). However, the total number of CD14, CD79 and CD2 infiltrated cells were lower in ACTH group than in control group (p