BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development.

DÍAZ, PABLO U.; HEIN, GJ.; BELOTTI, EM.; RODRIGUEZ, FM.; REY, F.; AMWEG, AYELEN N.; MATILLER, VALENTINA; BARAVALLE, EUGENIA; ORTEGA, HUGO H.; SALVETTI, NATALIA R.

REPRODUCTION

BIOSCIENTIFICA LTD

Lugar: Bristol; Año: 2016

1470-1626

Cystic ovarian disease (COD) is an importantcause of subfertility in dairy cattle. Bone Morphogenetic Proteins (BMPs),mainly BMP2, BMP4, and BMP6, play a key role in female fertility. In thisstudy, we hypothesized that an altered BMP system is associated with ovarianalterations contributing to COD pathogenesis. Therefore, we examined theexpression of BMP2, BMP4, BMP6 and BMP receptor 1B (BMPR1B) in the ovaries ofanimals with spontaneous or ACTH-induced COD, as well as during the developmentof the disease, in a model of follicular persistence induced by low doses ofprogesterone (at 5, 10 and 15 days of follicular persistence). Results showedchanges in BMP2, BMP4 and BMP6 expression during folliculogenesis, in granulosaand theca cells in the COD groups, as well as at the different stages offollicular persistence. Results also showed changes in BMPR1B expression indeveloping follicles in animals with COD, and at the initial stages offollicular persistence (P5). Comparison between groups showed significant differences,mainly in BMP4 and BMP6 expression, in granulosa and theca cells of different follicularcategories. The expression of these BMPs was also increased in cystic and persistentfollicles, in relation to antral follicles of the control group. BMPR1B showedhigh expression in cystic follicles. Together, these results may indicate analteration in BMPs, especially in BMP4 and BMP6, as well as in BMPR1B, whichoccurs early in folliculogenesis and incipiently during the development of COD,and which could be a major cause of recurrence of this disease in cattle.