Detection and activity of 11 beta hydroxylase (CYP11B1) in the bovine ovary.

AMWEG, AYELEN N.; RODRIGUEZ, FERNANDA M.; HUBER, EMILIA; MARELLI, BELKIS E.; GAREIS, NATALIA C.; BELOTTI, EDUARDO M.; REY, FLORENCIA; SALVETTI, NATALIA R.; ORTEGA, HUGO H.

REPRODUCTION

BIOSCIENTIFICA LTD

Lugar: Bristol; Año: 2017

1470-1626

Glucocorticoids (GCs) such as cortisol andcorticosterone are important steroid hormones with different functions inintermediate metabolism, development, cell differentiation, immune response andreproduction. In response to physiological and immunological stress,adrenocorticotropic hormone (ACTH) acts on the adrenal gland by stimulating thesynthesis and secretion of GCs. However, there is increasing evidence that GCsmay also be synthesized by extra-adrenal tissues. Here, we examined the geneand protein expression of the enzyme 11b-hydroxylase P450c11 (CYP11B1),involved in the conversion of 11-deoxycortisol to cortisol, in the differentcomponents of the bovine ovary and determined the functionality of CYP11B1 invitro CYP11B1 mRNA was expressed in granulosa and theca cells in small, mediumand large antral ovarian follicles, and CYP11B1 protein was expressed in mediumand large antral follicles. After stimulation by ACTH, we observed an increasedsecretion of cortisol by the wall of large antral follicles. We also observed aconcentration-dependent decrease in the concentration of cortisol in responseto metyrapone, an inhibitor of CYP11B1. This decrease was significant at10-5 µM metyrapone. In conclusion, this study demonstrated for the first timethe presence of CYP11B1 in the bovine ovary. This confirms that there could bea local synthesis of GCs in the bovine ovary and therefore a potentialendocrine responder to stress through these hormones.