ALLICIN NEUROPROTECTIVE EFFECT DURING OXIDATIVE/INFLAMMATORY INJURY INVOLVES AT1-Hsp70-iNOS COUNTERBALANCE AXIS

LUCIANA MAZZEI; MARÍA BELÉN RUIZ-ROSO; NATALIA DE LAS HERAS; SANDRA BALLESTEROS; CAROLINA TORRES; LEON FERDER; ALEJANDRA CAMARGO; MANUCHA, WALTER

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2020 vol. 44 p. 671 - 681

Abstract: The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest toelucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated aneuroprotective effect of allicin?a phytochemical present in garlic- linked to oxidative-inflammatory modulation.Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS)regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. Inconnection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However,there are no studies that evaluate AT1-Hsp70-iNOS interaction as a mechanism linked to neuroinflammation. Thus,our central aim is to evaluate if the allicin protective effect is associated with an AT1-Hsp70-iNOS counterbalanceaxis. For this study, a murine microglial cell line (BV-2) was injured with lipopolysaccharides and treated or not withallicin. Then, it was evaluated cell viability, proinflammatory cytokine levels, cellular oxidative stress, iNOS, Hsp70,and AT1 protein expression (cellular and mitochondrial fractions), nitrite levels, and protein-protein interactions. Theresults demonstrated that allicin could prevent neuronal injury due to a reduction in oxidative stress andinflammatory status mediated by an AT1-Hsp70-iNOS counterbalance axis linked to direct protein-protein interaction.