Role of IL-10 and its relationship with glucocorticoids in different endotoxic contexts that resemble to the sepsis phases

TODERO, MARÍA FLORENCIA; FONTANALS, ADRIANA; PINEDA, GONZALO; MONTAGNA, DANIELA; YOKOBORI, NOEMI; RAMOS, MARÍA VICTORIA; BARRIENTOS, GABRIELA; TOBLLI, JORGE; ISTURIZ, MARTÍN; REARTE, BÁRBARA

Congreso; SAIC SAI SAFIS 2018; 2018

In sepsis caused by Gram-negative infections the membrane lipopolysaccharides (LPS) have a central role, initially inducing an exacerbated production of pro-inflammatory cytokines followed by an anti-inflammatory state known as endotoxin tolerance (TOL). The inflammation resolution and TOL induction involves effectors as IL-10 and glucocorticoids (GC). The aim of this study was to evaluate the role of IL-10 in the pro/anti-inflammatory events induced by LPS and its relationship with GC. For this, wild-type (WT) BALB/c and IL-10 knockout (KO) mice were challenged with one doses of LPS (endotoxic shock) or inoculated daily with different doses of LPS (TOL). Corticosterone levels were determined by RIA, cytokines by ELISA, histopathology by periodic acid Schiff (PAS). After LPS challenge, WT showed high levels of plasma IL10 whereas it was not detectable (nd) in KO mice (IL-10 (pg/ml): WT= 6374±1505; KO= nd; p