ANALYSIS OF THE RUMEN BACTERIAL DIVERSITY OF CREOLE GOATS UNDER TWO DIFFERENT DIET CONDITIONS USINGq-PCR.

GRILLI, D.; KOPECNÝ, J.; MRÁZEK, J.; PAEZ LAMA, S.; EGEA, V.; CERON CUCCHI, M; DEGARBO, S.; TELECHEA, A.; ARENAS, N.

San Luis

Jornada; XXXII Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2014

Sociedad de Biología de Cuyo.

Quantitative real time (qPCR) assays was used to determine both the abundance and diversity of bacterial community characterizing rumen of Creole goats for which the diet was switched from alfalfa hay/corn (AH/C) to native forages (NF). Two fistulated goats were routinely fed with AH/C diet for a period of 21 days. Then, goats were switched to the NF diet for others 21 days. The ruminal contents were sampled at 2 (Day 0), 10 (Day 10) and 21 (Day 21) days after the beginning of each diet. One sample per animal was collected after feeding and was stored for later DNA extraction. Total bacteria, Firmicutes, Actinobacteria, Bacteroidetes and γ-Proteobacteria were quantified by qPCR using primers targeting 16S rRNA gene specific. In relation to diet, total ruminal bacteria were significantly higher in goats fed NF diet (11.75 ± 1.46 x 1010 bacteria g-1) than in those fed AH/C diet (6.39 ± 2.67 x 1010 bacteria g-1). Not significantly differences in concentrations of Firmicutes, Bacteroidetes or ɣ-Proteobacteria concentrations were observed during feeding experiment. However, Actinobacteria concentration was significantly higher in goats fed FN diet (24.65 ± 5.95 x 105 bacteria g-1) than in those fed AH/C diet (1.73 ± 0.28 x 105 bacteria g-1). Within this bacterial phylum, Actinomyces sp, Flavobacterium sp and Bifidobacterium sp have been described in the ruminal ecosystem, which could be selected at a high number in goats fed NF diet. Findings from q-PCR analysis suggest that rumen bacterial community of Creole goats was influenced by diet. New bacterial species have been associated with the degradation of the nutritional components in diets in the rumen of these animals.enviar mensaje