Seasonal variation of plant defense inductor ellagitannins in strawberry leaves under field conditions for phytosanitary technological applications

DI PETO, PÍA DE LOS ÁNGELES; CARLOS FROILÁN GRELLET BOURNONVILLE; CERVIÑO ANA MARÍA; CASTAGNARO, ATILIO P.; SCHMEDA-HIRSCHMANN, GUILLERMO; DIAZ-RICCI, JUAN CARLOS; MAMANÍ, ALICIA; FILIPPONE, MARÍA PAULA

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2021

0021-8561

Many natural compounds can activate the plant immunity and for that reason acquired special interest in crop disease management. Previously, we isolated from strawberry leaves a novel ellagitannin (HeT) which elicits plant defense responses. In this study we investigated bioactive compounds capable to induce defense responses in Arabidopsis thaliana against a bacterial pathogen, from field collected strawberry leaves. Methanolic extracts of strawberry leaves sampled at different months were obtained and compared. Higher content of total soluble phenolic compounds was found in methanolic extracts of leaves sampled in December (DME). Defense response induced in A. thaliana by DME was attributed to two ellagitannins, the HeT, and a new one, a galloyl-HHDP-glucose. Both compounds exhibited phyto-protective effects against Pseudomonas viridiflava and induced the expression of PDF1.2 and PR1 genes. These results provide economic value to strawberry leaves, normally discarded at the end of crop period, as a raw material for plant health enhancer bioinputs.