A novel on-line preconcentration method for trace molybdenum determination by USN-ICP OES with bio-sorption on immobilized Yeasts

RAUL A. GIL; SERGIO PASINI-CABELLO; ANDRÉS TAKARA; PATRICIA SMICHOWSKI; ROBERTO A. OLSINA; LUIS D. MARTÍNEZ

MICROCHEMICAL JOURNAL

ELSEVIER

Lugar: Amsterdam ; Año: 2007 p. 1 - 20

0026-265X

Abstract A new system for on-line preconcentration of molybdenum by sorption on a minicolumn associated to inductively coupled plasma — optical emission spectrometry with ultrasonic nebulization was studied. It is based on the sorption of molybdenum on a column packed with immobilized baker´s yeasts on controlled pore glass without further complexing reagent. The molybdenum preconcentrated by biosorption was subsequently eluted with hydrochloric acid. Considering a sample flow rate of 5.0 mL min−1, 10 mL of sample was preconcentrated in 2 min — optical emission spectrometry with ultrasonic nebulization was studied. It is based on the sorption of molybdenum on a column packed with immobilized baker´s yeasts on controlled pore glass without further complexing reagent. The molybdenum preconcentrated by biosorption was subsequently eluted with hydrochloric acid. Considering a sample flow rate of 5.0 mL min−1, 10 mL of sample was preconcentrated in 2 min −1, 10 mL of sample was preconcentrated in 2 min achieving asensitive total enhancement factor of 480-fold, and the detection limit (3 s) obtained was 21 ng L−1. Additionally, the calculated precisions expressed as percent relative standard deviation (RSD%) was 1.9%. Satisfactory results were obtained for the determination of molybdenum in standard reference material NIST 1643e Trace Elements in Water and real water samples. © 2007 Elsevier B.V. All rights reserved. Satisfactory results were obtained for the determination of molybdenum in standard reference material NIST 1643e Trace Elements in Water and real water samples. © 2007 Elsevier B.V. All rights reserved.