Analyzing ribosomal proteins as part of the potato defensome

D IPPÓLITO, S. ; SALCEDO, F. ; CASALONGUÉ, C. AND GODOY, V.

Mar del Plata, Buenos Aires

Congreso; Sociedad Argentina de investigación Bioquímica y Biología Molecular (SAIB); 2007

Sociedad Argentina de investigación Bioquímica y Biología Molecular (SAIB)

Abstract In plants, an essential step in the study of the induced defense response against environmental stresses is to describe the global changes that occur at the transcriptional level. In our laboratory, approximately 460 cDNA clones were obtained from a differential screening of a cDNA library from F. eumartii-infected potato tubers. These cDNA potato clones were classified in different functional categories. In order to extend the study of these cDNAs we undertook an in silico expression analysis by using microarray data available at TIGR. In particular, 44 cDNAs belonging to different functional categories were analyzed under different experimental conditions, including biotic and abiotic stresses. A total of 28 cDNAs were specifically up-regulated under biotic stress conditions. Among them, 7 potato cDNAs encode for ribosomal proteins. Extraribosomal functions of the ribosomal proteins have been postulated. However, their roles in the plant defense response are still discussed. In conclusion, the in silico and experimental expression analysis allowed us to select them as a set of genes with potential interest to further study of the plant defense response. Supported by UNMdP, ANPCyT and CONICET.