EVIDENCES OF POSTTRANSCRIPTIONAL CONTROL DURING THE DEFENSE RESPONSE OF POTATO TUBERS

D HIPÓLITO, S; SALCEDO, MF; CASALONGUÉ, CA; GODOY

Villa Carlos Paz, Córdoba, Argentina

Congreso; Sociedad Argentina de investigación Bioquímica y Biología Molecular (SAIB); 2008

Sociedad Argentina de investigación Bioquímica y Biología Molecular (SAIB)

Abstract One important disease of the potato (Solanum tuberosum) tubers is the dry rot caused by Fusarium species. In our lab, around 500 cDNAs were identified after the differential screening of a potato tuber library inoculated with F. eumartii. Here we report the expression analysis of 358 cDNAs from the collection using DNA macroarray technology. Changes in gene expression of potato tubers wounded or wounded and inoculated with F. eumartii at 0 and 24 hs post-treatments were monitored. About 85% of the cDNAs were induced at least two-fold after the treatments.150 of these cDNAs were only induced by F. eumartii at 24 hs post inoculation. Infected tubers specifically accumulated transcripts encoding amino acid metabolism enzymes involved in nitrogen recycling and mobilization. The mobilization of nitrogen might be a strategy used by the fungus to ensure successful colonization. In addition, F. eumartii induced differential abundance of transcripts for several proteins involved in RNA processing and export. These genes have not been related to the potato defense response and belong to different families of RNA-binding proteins. Three of these cDNAs showed conserved RNA helicase domains. These results contribute to the identification of new candidate genes that might regulate potato tuber responses against F.eumartii at the posttranscriptional level. Supported by UNMdP, ANPCyT and CONICET