Relevance of IFN-gamma and IL10 induced by probiotic fermented milk administration in the regulation of allergies development

VELEZ, E.M.; BIBAS BONET, M.E.; CARMUEGA, E.; WEIL, R.; PERDIGÓN, G.; MALDONADO C.

Carovigno

Workshop; 7th International Immunonutrition Workshop; 2014

Different studies have shown the ability of probiotics to reduce and regulate immuneresponse during the allergic development processes (1) (2) (3). The aim of this work was toevaluate the potential efficacy of a probiotic fermented milk (PFM) in the prevention ofallergy development and to investigate the mechanisms involved in a mouse model.Ovoalbumin (OVA) sensitized adult BALB/c mice were administered before sensitizationor continuously with a PFM: Normal-Control (NC), Basal (B-5days-PFM); OVASensitization-Control (SC), Previous (P-5d PFM+OVA+H2O) and Continuous (C-5dPFM+OVA+PFM) treatment. At 7 and 15 days post-sensitization (dPS) and 2 days post-restimulus (dPR) we studied in bronchoalveolar lavage fluid (BALF): specific-IgE, IgG,total-S-IgA. In macerated lung tissue: IL10, IL4, INFγ. In serum: specific-IgE, IgG, IgG1,IgG2a, IL4 and IL10 (by ELISA). In lung tissue IL4+, IL10+, INF-γ+, CD4+, CD8+ andF4/80+ cells by immunofluorescence.Results showed that PFM significantly reduced specific IgE in serum and BALF at 7 dPS(p≤0.05) (fig. A). There was no alteration of the normal response to a-OVA-IgG and totalS-IgA. The main cytokines up-regulated by PFM were INF-γ (7 and 15 dPS, and 2DPR)and IL10 (7dPS) (p≤0.05) (Fig. B and C). IL-4 had no differences between studied groupsin lungs or serum.We found that PFM intake induces mainly a Th1 response. This could be throughcytokines induction like Il-10 and IFN-γ produced in lungs from mice with a continuoustreatment. There is also a surveillance state in lungs with increased total S-IgA induced byPFM, reinforcing epithelial barrier (as seen in previous work to the gut level). Resultsindicate that the immune response found is mainly a local response in the lungs mucosamediated by INF-γ and IL-10 that controlled the IgE synthesis. There still a regulatoryeffect that must be studied that could involve mast cells or Treg cells1-Ouwehand AC (2007) Antiallergic effects of probiotics. J Nutr 137, 794S-797S2-Ishida Y, Bandou I, Kanzato H et al. (2003) Decrease in ovalbumin specific IgE of mice serum afteroral uptake of lactic acid bacteria. Biosci Biotechnol Biochem 67, 951-9573-Ohno H, Tsunemine S, Isa Y et al. (2005) Oral administration of Bifidobacterium bifidum G9-1suppresses total and antigen specific immunoglobulin E production in mice. Biol Pharm Bull 28,1462-1466.