The PAMPA Datasets: A metagenomic survey of microbial communities in Argentinean pampean soils Journal: Microbiome

RASCOVAN, NICOLAS; CARBONETTO, MARIA BELÉN; SANTIAGO REVALE; MARINA D REINERT; ALVAREZ, ROBERTO; GODEAS, ALICIA M.; COLOMBO, ROXANA P.; AGUILAR, MARIO; IANNONE, LEOPOLDO; NOVAS, VICTORIA; ZELADA, ALICIA; PARDO, ALEANDRO; SCHRAUF, GUSTAVE; MENTABERRY, ALEJANDRO; VAZQUEZ, MARTIN P

MICROBIOME

BioMed central - Springer

Lugar: Londres; Año: 2013 p. 1 - 21

2049-2618

Soil is among the most diverse and complex environments in the world. Soil microorganisms play an essential role in biogeochemical cycles and affect plant growth and crop production. However, our information of the relationship between species-assemblies and soil ecosystem processes is still scarce. The aim of this study was to generate a comprehensive metagenomic survey to evaluate the effect of high input agricultural practices on soil microbial communities. We collected soil samples from five different sites in Argentinean Pampaean region under three different types of land uses and two soil sources (bulk and rhizospheric). We obtained total DNA from all samples and, in addition, cDNA from rhizospheric samples. We generated 112 16S rDNA and 14 16S rRNA amplicon libraries totaling 1.3 Mi reads and 36 shotgun metagenome libraries totaling 17.8 Mi reads (7.7 Gb) using 454-FLX technology. Our preliminary results, based on the metadata and datasets combined, suggested that water availability was a primary driver defining microbial assemblages over land use and soil source when annual precipitation was below 800 mm. However, land use was a primary driver when water was not a limiting resource. These datasets are the first soil metagenomic study conducted in Argentina and one of the few large soil datasets available. The detailed analysis of these data will be a step forward in the comprehension of soil microbiome response to high-input agricultural systems, and they will serve as a useful comparison with other soil metagenomic studies worldwide.