NANO-FORMULATED ANANDAMIDE DECREASES NEUROINFLAMMATORY MARKERS IN SPONTANEOUSLY HYPERTENSIVE RATS.

FERES JOSÉ MOCAYAR MARÓN; VIRNA MARGARITA MARTÍN GIMÉNEZ; LUCIANA MAZZEI; EMILIANO DIEZ; SEBASTIÁN GARCÍA; MANUEL GUEVARA; DIEGO ENRIQUE KASSUHA; ROBERTO YUNES; WALTER MANUCHA

Córdoba

Congreso; XXXIII Congress of the Argentine Society for Research in Neuroscience; 2019

Essential hypertension is responsible for almost 95% of all cases of hypertension. Frequently of neurogenic origin, it is linked with an over-excitation of brainstem centres, sympathetic hyperactivation and imbalance in the levels of pro- and anti-inflammatory cytokines. Spontaneously hypertensive rats (SHR) is a validated model of hypertension, plus several neurocognitive deficits. Since endocannabinoid anandamide (AEA) protects neurons from the inflammatory damage, and cannabinoid signalling decreases in brains of hypertensive animals, we applied a nano-formulated AEA in SHR. We used adult male rats (n=7) of 250-300 g normotensive (WKY) and hypertensive (SHR), treated or not with nano-formulated AEA in polycaprolactone (AEA/PCL), at a weekly dose of 5 mg/Kg IP, for four weeks. Regarding WKY, the SHR showed elevated inflammatory markers (IL-1, IL-6, FNTα, ultrasensitive PCR and plasma Hsp70, p