REGULATION OF ERYTHROFERRONE EXPRESSION BY ERYTHROPOIETIN IN ERYTHROID CELLS

VILLAROSA, JULIETA; CHAMORRO, MARÍA EUGENIA; MACIEL PACCINI, JUAN IGNACIO; NESSE, ALCIRA; VITTORI, DANIELA; MALTANERI, ROMINA EUGENIA

Mar del Plata

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2022

SAIC-SAI-FAIC-SAFIS

The recently characterized protein Erythroferrone (ERFE), produced by erythroid precursors, is known to reduce Hepcidin (Hep) levels in hepatocytes, allowing the release of intracellular Fe into the bloodstream. Previous research proposed ERFE as an indirect mediator of Erythropoietin (Epo) in hepatocytes. This mechanism plays a key role in the regulation of Fe availability for erythropoiesis. With the aim of studying the mechanism through which Epo induces ERFE expression, erythroleukemic K562 cells were treated with Epo (80 ng/mL). ERFE expression was significantly induced by Epo after 1 h, and returned to baseline levels after 24 h (RT-qPCR, a.u.: t0=1, *t1h=3.68±1.2, t24h=0.98±0.24, *p