Molecular characterization of Lotus tenuis with contrasting behavior for salinity

ACUÑA, MARIELA; MACIEL, MARIA; AFFINITO, AGOSTINA; ANDRÉS, ADRIANA

Utah

Simposio; The 7th International Symposium on the Molecular breeding for the genetic improvement of forage crops and turf; 2012

MBFT

In Argentina over the past years there was a strong increase of agricultural production displacing the livestock production to marginal areas. One of the species more relevant to these environments is Lotus tenuis Waldst. et Kit, perennial legume, diploid 2n = 2x = 12, allogamous, with a spring-summer-autumn production. This species is naturalized in the fields of Flooding Pampa, valued for its contribution to forage supply of livestock systems in the region. The characterization of genetic variability of L. tenuis has been based on the description of morpho-physiological traits, recently have been incorporated biotechnology tools such as molecular markers (MM) they are widely used in breeding programs as it provides more direct and predictive techniques. The aim of this work was to conduct molecular characterization of two synthetic materials over L. tenuis, with contrasting behavior for salinity (tolerant vs. susceptible) according to preliminary researches. Were used four microsatellite (SSR) from L. japonicus and the transference was performed making the adjustments to achieve it. Like melting temperature according to the sequences of each primer, DNA quantity, concentration of magnesium chloride, cyclized program, etc..Thirty seedlings were analyzed for each synthetic material, six bulks each one with five seedlings. In this way was analyzed the presence/absence of the bands/alleles in each bulk, obtaining a 27% more of bands/alleles in susceptible bulks than the tolerant ones. These results not only allowed to generate basic knowledge on the transfer of SSR from L. japonicus to L tenuis, but also apply and integrate the use of MM in breeding programs of the specie and to start the searching for MM associated with salinity tolerance.