A preliminary linkage map in pea (pisum sativum l.) Based on srap markers (sequence-related amplified polymorphism)

GUINDÓN, MARÍA FERNANDA; ZAYAS, ALDANA; MARTIN, EUGENIA; CRAVERO, VANINA; COINTRY, ENRIQUE

Rosario

Congreso; XV Congreso y XXXIII Reunión Anual - Año 2013; 2013

Facultad de Ciencias Agrarias de la Universidad Nacional de Rosario

A mapping population of Pea was derived from a cross between the cultivars Zav 25 and DDR11 with a view to generating a new genetic linkage map of Pisum sativum L. based on SRAP markers. Fifteen primer SRAP combinations were used to detect 183 polymorphic bands (12,2 polymorphic bands/primer combination). The most polymorphic primer combination was Me3-Em2, with 29 polymorphic bands. Fragments generated by SRAP were treated as dominant markers. Goodness-of-fit between the observed and expected segregation ratios was determinate by a Chi-square test. Markers without a significant level of segregation distortion (χ2> χ2α=0.01) were used to construct the map on JOIN MAP v4 software. The linkage map consists in 50 genetic markers distributed on 7 linkage groups (LGs) covering a total of 310,5 cM. The LGs length ranging from 20.4 to 80.8 cM (mean 44,29 cM) and including 4-10 markers. The 75 unlinked markers reflect the need to include a large number of markers to cover the entire genome. SRAP Primers Sequence: Me3: 5´-TGAGTCCAAACCGGAAT-3´ Em2: 5´-GACTGCGTACGAATTTGC-3´